Dr. Amit Singh

Contact Info
Dr. Amit Singh
8505808200
Department of Microbiology, Central University of Punjab, Bhatinda
Dr. Amit Singh

Assistant Professor

Department of Microbiology

School of Basic Sciences

Qualification

 Educational Qualifications

Degree

 Institution/ University

Year

Subject/Work

PhD

All India Institute of Medical Sciences (AIIMS), New Delhi, India

2008-2016

Clinical Microbiology & Molecular Medicine

MSc.

Jiwaji University, Gwalior, (MP), India

2004-2006

Clinical Microbiology

BSc.

A.P.S. University, Rewa (MP), India

2001-2003

Zoology, Botany and Chemistry
Experience
Teaching & Research Experience
Position Held Place of Work Start Date End Date
Assistant Professor  Department of Microbiology, Central University of Punjab, Bhatinda  Nov, 2022 till date
Scientist II All India Institute of Medical Sciences, New Delhi Feb, 2020 Nov, 2022
Senior Resident All India Institute of Medical Sciences, Bhopal, Madhya Pradesh Jan, 2019 Jan, 2020
Research Officer All India Institute of Medical Sciences, New Delhi May, 2016 Dec, 2018
SRF-ICMR All India Institute of Medical Sciences, New Delhi May, 2009 Dec, 2013
JRF All India Institute of Medical Sciences, New Delhi Jan, 2007 April, 2009
Administrative Experience

Member Secretary-Institutional Biosafety Committee (IBSC), CUP

Faculty-In-Charge, Centralized Microbial Facility & BSL2 facility, CUP

Member, Board of Studies, CUP

Member, Institute Innovation Council (IIC), CUP

Convener, Academic and Administrative Committee (AAC)

Model Co-ordinator, Virtual Lab Programme

CCSEA Nominee

Any Other Experience
Research Area
  • Infection biology
  • Proteomics 
  • Cloning expression and purification of proteins
  • Nanobiosensor, electrochemical sensor, nanomedicine etc.
  • Point-of-Care test,  ICT-based rapid test 
  • Genomics, WGS, NGS
  • Antimicrobial resistance
  • Generation of monoclonal and polyclonal antibodies
  • Molecular diagnosis of infectious disease and Respiratory viruses.
  • Epidemiological studies, 
  • Research Work
    Identification of biomarkers and development of POC test in different format using antigens, mAb, biosensors. 
Research Interests
  • Drug-protein interaction
  • Screening and selection of novel antimicrobial active compounds against infectious disease from Indian herbs.
  • Estimating toxicity and genotoxicity of various synthetic and natural drugs in various cell lines.
  • Development of diagnostics for infectious disease (molecular diagnosis and Point-of-care test).
  • Molecular epidemiology
  • Elucidating drug resistance mechanisms, Antimicrobial resistance among ESKAPE pathogens and tuberculosis.
  • Identifying and characterizing novel proteins/antigens for serodiagnosis of MDR/XDR tuberculosis, ESKAPE pathogens.
  • Proteomics, genomics and transcriptomics of infectious disease.
  • Translational research.\
  • Nanobiosensor and nanomedicine.
  • Hybridoma technology, monoclonal and polyclonal antibodies
  • Bioinformatics analysis for drug-protein interaction, protein-protein interaction, and ABC transporter of Mycobacterium 
  • Elucidation drug resistance mechanisms of M. tuberculosis

    Figure: Elucidation the drug resistance mechanisms of M. tuberculosis against first line and second line ATT.

Open to collaborating for research work in any of the above fields or based on technical expertise, 

Research Grants/Projects
  1. Detection of a triacetylfuasarinine C in serum and urine by LC-MS/ MS as a potential biomarker of invasive aspergillosis” (2021-2022) AIIMS Intramural funds (Rs. 10 Lakh) as Co-PI.
  2. Identification, cloning, purification and characterization of proteins, involved in multi-drug resistance in Mycobacterium tuberculosis” funded by Indian Council of Medical Research (ICMR), Govt. of India from 2010-2013.
  3. Development and evaluation of a rapid point‐of‐care test for the early diagnosis of tuberculosis from patient blood samples, CUP, RSM grants (PI)
Professional Affiliation

Life Member & Member 
1. Asian-African Society of Mycobacteriology (AASM), Membership 
2. International Society of Infectious Disease (ISID).
3. American Peptide Society 
4. Life member of Asia Society of Researchers 
5. APMS. 
6. AIIMSONIAN 
7. Society of Young Scientists (SYS).
8. Advisor of the Indian Scientific Education and Technology Foundation (ISETF). 
9. American Association of Microbiologists (ASM)

Professional Recognition/Awards/Scholarships
  1. Dr. Tulsidas Chugh Award (2019) at NAMSCON-2019, AIIMS, Bhopal, India
  2. LMCE Travel Grand Award to attend the LMCE 2018 (Laboratory Medicine Congress & Exhibition) & KSLM 59th annual meeting in Seoul, South Korea dated November 1, 2018, to November 3, 2018.
  3. International Travel Award DBT-CTEP  to attend the LMCE 2018 in Seoul, South Korea.
  4. AIIMS Excellence Research Award 2017 titled “Evaluation of 5 novel protein biomarkers for the rapid diagnosis of pulmonary and extra-pulmonary tuberculosis: preliminary results”.
  5. Young Scientist Award by Bill & Melinda Gates Foundation for MICROBE 2016 at Boston, USA.
  6. International Travel Support from the Department of Biotechnology (DBT) and SERB, Govt. of India to attend the conference ICAAC/ICC 2015 at San Diego, California, USA.
  7. Young Scientist Award, FEMS 2013, Leipzig, Germany.
  8. Young Scientist Travel Award from ICMR to attend FEMS 2013 at Leipzig, Germany.
  9. Young Scientist Travel Award from to attend FEMS 2013 at Leipzig, Germany.
  10. Senior Research Fellowship (SRF) From Indian Council of Medical Research, Govt. of India from Dec. 2010 to Dec. 2013.
  11. Best Poster Award (Immunology Foundation, New Delhi) in International Conference on Opportunistic Pathogens in AIDS (ICOPA-2008) January 27th – 29th 2008 at All India Institute of Medical Sciences, New Delhi, India.
  12. Best Poster Award in IX Sir Dorabji Tata Symposium (antimicrobial resistance) at National Institute of Advanced Studies, Indian Institute of Science, Bangalore, India on 10-11th March 2008.
  13. International Travel Award from Department of Biotechnology (DBT), Govt. of India to participate in FEMS 2009 International conference, held in Gothenburg, Sweden from 28th June to 2nd July 2009.
  14. Elected as a President of Student Union in Govt. New Science College, APS University, Rewa (M.P.) in the year 2002-2003.
  15. Secured merit rank in B.Sc. 2nd Year at Govt. New Science College, APSU, Rewa MP. in 2002-2003.
Research Collaboration

1. AIIMS, New Delhi

2. AIIMS, Bhopal

3. AIIMS, Bathinda

4. ICGEB, New Delhi

5. Lady Hardinge Medical College

6. MRU, Maulana Azad Medical College, New Delhi

7. National JALMA Institute of Leprosy Research and Other Mycobacterial Disease.

8. Alagappa University, Tamilnadu

9. Acharya Institute of Technology, Bangalore

10. JIPMER, Puducherry

 

OPEN TO COLLABORATING FOR RESEARCH

Publications
  1. Sharma J, Sharma D, Singh A, Sunita K. (2022). olistin Resistance and Management of Drug Resistant Infections. Can J Infect Dis Med Microbiol. 10;2022:4315030. (IF 2.8).
  2. Malhotra S, Kalaivani M, Lodha R, Bakhshi S, Mathur VP, Gupta P, Kedia S, Sankar MJ, Kumar P, Kumar A, Vikas H, Ahuja V, Sinha S, Guleria R, Dua A, Ahmad S, Upadhyay AD, Sati HC, Mani K, Lokade AK, Devi KP, Johnson RM, Gowthaman K, Kumari M, Singh R, Kalra D, Vasudha S, Sharma S, Singh A,  et al., (2022). COVID-19 Infection, and Reinfection, and Vaccine Effectiveness Against Symptomatic Infection Among Health Care Workers in the Setting of Omicron Variant Transmission in New Delhi, India. Lancet Reg Health Southeast Asia, 6;100023. doi: 10.1016/j.lansea.2022.100023.
  3. Katiyar AS, Singh A, Abhishek A, Bhaskar M, Sharma D, Garg V. Phytoextraction of chromium, iron and nickel by Indian Rice Plant (Oryza sativa L.): An opportunity for management of multi-metal contaminated tannery wastewater. J of Exp Biol and Agri Sci. 10 (3), 1-20.
  4. Gupta A, Khurana S, Das R, Srigyan D, Singh A, Mittal A, Singh P, Soneja M, Kumar A, Singh AK, Soni KD, Meena S, Aggarwal R, Sharad N, Aggarwal A, Kadnur H, George N, Singh K, Desai D, Trilangi P, Khan AR, Vandana V Kiro VV, Naik S, Arunan B, Goel S, Patidar D, Lathwal A, Dar L, Trikha A, Pandey RM, Malhotra R, Guleria R, Mathur M, Wig N. (2020). Rapid chromatographic immunoassay-based evaluation of COVID-19: A cross-sectional, diagnostic test accuracy study and its implications for COVID-19 management in India. Indian J Med Res. 153 (1), 126. (IF- 5.274).
  5. Bhattacharya B, Kumar R, Meena VK, Singh A, Das R, Xess A, Arif N, Soneja M, Vig S, Rastogi V, Bhatnagar S, Mohan A, Dar L, Wig N. (2020). SARS-CoV-2 RT-PCR profile in 298 Indian COVID-19 patients: a retrospective observational study. Pathogens and Disease 78 (7). (IF-3.951).
  6. Batra P, Singh A, Ibal A, Singh S (2018). “The end TB strategy: where do we stand still in India”. EC Pulmonology and Respiratory Medicine 8.1: 01-02.
  7. Rufai SB, Singh A, Nigam K, Sharma D, Gupta AK, Singh J, Kumar P, Sankar MM, Rawat VS, Singh S. (2017). Diagnostic usefulness of Xpert MTB/RIF assay for detection of tuberculous meningitis using cerebrospinal fluid. J Infect. S0163-4453(17)30130-5. doi: 10.1016/j.jinf.2017.04.010. (IF- 38.637).
  8. Singh A, Gupta AK, Gopinath K, Sharma P, Singh S. (2017). Evaluation of 5 novel protein biomarkers for the rapid diagnosis of pulmonary and extra-pulmonary tuberculosis: preliminary results. Sci. Rep. 7, 44121; doi: 10.1038/srep44121. (IF-4.9996).
  9. Srivastava S, Mishra J, Gupta AK, Singh A, Shankar P, Singh S. (2017) Laboratory confirmed miltefosine resistant cases of visceral leishmaniasis from India. Parasite & vector. 10:49. DOI 10.1186/s13071-017-1969-z (IF- 4.047).
  10. Singh A, Gupta AK, Gopinath K, Sharma D, Sharma P, Bisht D, Sharma P, Singh S. (2016). Comparative proteomic analysis of sequential isolates of Mycobacterium tuberculosis sensitive and resistant Beijing type from a patient with pulmonary tuberculosis. Int. J. Mycobacteriol. http://dx.doi.org/10.1016/j.ijmyco.2016.10.028. (IF-0.271).
  11. Gupta AK, Srivastava S, Singh A, Singh S. (2016). Development of rapid immuno-diagnostic test for the early detection of tuberculosis. Int. J. Mycobacteriol. http://dx.doi.org/10.1016/j.ijmyco.2016.11.008. (IF-0.271).
  12. Rufai SB, Singh S, Singh A, Kumar P, Singh J, Vishal A. (2017). Performance of Xpert MTB/RIF on ascitic fluid samples for detection of abdominal tuberculosis. J Lab Physicians. 9(1), 47.
  13. Singh S, Singh A, Mukherjee A, Prajapati S and Delhi Pediatric TB study group, Singh V, Kabra SK, Hesseling AC. (2015). Performance of Xpert MTB/RIF on archived gastric aspirate and induced sputum samples from children with probable intrathoracic tuberculosis. BMC Microbiology 15:191. (IF- 3.605).
  14. Rufai SB, Singh A, Kumar P, Singh J, Singh S. (2015). Performance of Xpert MTB/RIF in the diagnosis of pleural tuberculosis from a TB high burden setting. J. Clin. Microbiol. 53;11 3636-3638. (IF- 11.677).
  15. Gupta AK, Srivastava S, Singh A, Singh S. (2015). De Novo whole genome sequence and annotation of Leishmania strain isolated from a case of post-kala-azar dermal-leishmaniasis. Genome Announc. 3(4): e00809-15. (IF- 0.19).
  16. Singh A, Gopinath K, Sharma P, Bisht D, Sharma P, Singh N,  Singh S. (2015). Comparative proteomic analysis of sequential isolates of Mycobacterium tuberculosis from a patient pulmonary tuberculosis turning from drug sensitive to multi drug-resistant. Indian J Med Res. 141(1) 27-41. (IF- 5.274).
  17. Lagrange PH, Thangaraj SK, Dayal R, Deshpande A, Ganguly NK, Girardi E, Joshi B, Katoch K, Katoch VM, Kumar M, Lakshmi V, Leportier M, Longuet C, Malladi SV, Mukerjee D, Nair D, Raja A, Raman B, Rodrigues C, Sharma P, Singh A, Singh S, Sodha A, Kabeer BS, Vernet G, Goletti D. (2014). Toolbox for Tuberculosis (TB) Diagnosis: An Indian Multi-Centric Study (2006-2008); Evaluation of Serological Assays Based on PGL-Tb1 and ESAT-6/CFP10 Antigens for TB Diagnosis. PLoS One. 5;9(5):e96367. doi: 10.1371/journal.pone.0096367. (IF -3.752).
  18. Singh A, Gopinath K, Singh N, Singh S. (2014). Deciphering the sequential events during in vivo acquisition of drug resistance in Mycobacterium tuberculosis. Int J Mycobacteriol. 3;36-40. doi:10.1016/j.ijmyco.2013.10.006. (IF-0.271).
  19. Rufai SB, Kumar P, Singh A, Prajapati S, Balooni V, Singh S. (2014). Comparison of Xpert MTB/RIF with line probe assay for detection of rifampicin mono resistant Mycobacterium tuberculosis. J Clin Microbiol. 52(6):1846-52. doi: 10.1128/JCM.03005-13. (IF- 11.677).
  20. Lagrange PH, Thangaraj SK, Dayal R, Deshpande A, Ganguly NK, Girardi E, Joshi B, Katoch K, Katoch VM, Kumar M, Lakshmi V, Leportier M, Longuet C, Malladi SV, Mukerjee D, Nair D, Raja A, Raman B, Rodrigues C, Sharma P, Singh A, Singh S, Sodha A, Kabeer BS, Vernet G, Goletti D. (2013). A toolbox for tuberculosis (TB) diagnosis: an indian multicentric study (2006–2008). Evaluation of Qu    antiFERON-TB Gold in tube for TB diagnosis. PLoS One. 6;8(9):e73579. doi: 10.1371/journal.pone.0073579. (IF -3.752).
  21. Kumar P, Singh A, Sharma U, Singh D, Dobhal MP, Singh S. (2013). Anti-mycobacterial activity of plumericin and isoplumericin against MDR Mycobacterium tuberculosis. Pulm Pharmacol Ther. doi:pii: S1094-5539(13)00005-9. 10.1016/j.pupt.2013.01.003. (IF- 3.410).
  22. Lagrange PH, Thangaraj SK, Dayal R, Despande A, Ganguly NK, Girardi E, Joshi B, Katoch K, Katoch VM, Kumar M, Lakshmi V, Leportier M, Longuet C, Malladi SV, Mukerjee D, Nair D, Raja A, Raman B, Rodrigues C, Sharma P, Singh A, Singh S, Sodha A, Kabeer BS, Vernet G, Goletti D. (2012). A toolbox for tuberculosis diagnosis: an indian multicentric study (2006-2008): microbiological results. PLoS One. 7(8):e43739. doi: 10.1371/journal.pone.0043739. (IF- 3.752).
  23. Sankar MM, Kumar P, Munawwar A, Kumar M, Singh J, Singh A, Parashar D, Malhotra N, Duttagupta S, Singh S. (2012). The usefulness of multiplex PCR in the diagnosis of genital tuberculosis in females with infertility. Eur J Clin Microbiol Infect Dis. 32(3):399-405. doi: 10.1007/s10096-012-1755-y. (IF- 5.103).
  24. Sharma D, Sharma J, Singh A. (2021). Exploring the mystery of angiotensin-converting enzyme II (ACE2) in the battle against SARS-CoV-2. J Renin Angiotensin Aldosterone Syst. Volume 2021, Article ID 9939929, 5 pages. (IF-4.109).
  25. Gupta AK, Singh A, Singh S. (2014) Glycogenomics of Mycobacterium tuberculosis. Mycobact Dis 4:175. (IF- 0.764).
  26. Gupta AK, Singh A, Srivastava S, Shankar P, Singh S. (2016). Visceral leishmaniasis in children: diagnosis, treatment and prevention. J Pediatr Infect Dis doi https://doi.org/10.1055/s-0037-1603500. (IF-3.806).
  27. Singh A, Gupta AK, Sharma D. (2022). Mechanisms of biofilm-based antibiotic resistance and tolerance in Mycobacterium tuberculosis" in book entitled “Bacterial Survival in the hostile environment” First Edition published by Elsevier. ISBN: 9780323918060 (in press).
  28. Gupta AK, Animesh S, Singh A. (2022) An overview of immunosensors and their application. (Accepted) ‘Biomaterials-Based Sensors’ Springer Nature.
  29. Gupta AK, Sharma D, Singh A (2021). Proteomics of Mycobacterium tuberculosis: An overview. Volume 1-Current and future development in proteomics; Microbial proteomics: development in technologies and applications edited by Sharma D. (ISBN 978-981-14-9141-2). Bentham Science Publishers
  30. Singh A, Gupta A.K, Singh S. (2019). Molecular mechanisms of drug resistance in M. tuberculosis: Role of nanoparticles against MDR-TB. Nano-Biomedicine, Edited by SK Saxena, SM Paul Khurana and RK Gaur. (ISBN 978-981-32-9897-2). Springer-Nature.
  31. Gupta AK, Singh A, Singh S. (2019). Diagnosis of tuberculosis: nano-diagnostics approaches, in NanoBio-Medicine, Edited by SK Saxena, SM Paul Khurana and RK Gaur. (ISBN 978-981-32-9897-2). Springer-Nature. Authors contributed equally..
  32. Gupta AK, Singh A, Singh S. (2017). Glycogen as key energy storehouse and possibly responsible for multidrug resistance in Mycobacterium tuberculosis. Drug resistance in bacteria, fungi, malaria, and cancer. Arora G, Sajid A, Kalia VC. (ISBN 978-3-319-48683-3). Springer Nature.
  33. Singh A, Sharma D, Gopinath K, Sharma P, Bisht D, Sharma P, Singh S. Beijing type Mycobacterium tuberculosis: A comparative proteomic analysis of sequential clinical isolates isolated from a patient with pulmonary tuberculosis. Expert Reviews of Proteomics (in revised review).
  34. Gupta AK, Singh A, Srivastava S, Sharma P, Singh S. (2020). Generation of monoclonal antibodies against novel recombinant antigens of Mycobacterium tuberculosis and its evaluation for the diagnosis of active tuberculosis. (Submitted in Cell Heliyon).
  35. Sharma D, Sharma J, Sharma S, Singh A, Dhuriya YK. (2022). Tuberculosis might potentiate the severity of COVID-19 and their probable management in developing countries. (Submitted in Frontiers in Medicine).
  36. Srivastava S, Gupta AK, Singh A, Singh S. (2020). Leishmania kinesin motor domain with BCG, as a vaccine formulation against experimental visceral leishmaniasis. (Submitted in Frontiers in Cellular and Infection Microbiology).
  37. Katiyar AS, Bhaskar M, Singh A, Sharma D, Garg V. Biophysical and biochemical characterization of untreated tannery effluent and treated tannery effluent from common effluent treatment plant enriched by microbial mixture. (Submitted in Phytoremediation).
  38. Singh A. Antimicrobial Susceptibility and Plasmid Profiles of Escherichia coli isolates from Patients with Urinary Tract Infection. (Submitted in Canadian Journal of Infectious Disease and Medical Microbiology).
  39. Sharma J, Sharma D, Singh A. (2022). Colistin resistance and management of drug resistant infections. (Submitted in Canadian Journal of Infectious Disease and Medical Microbiology).
  40. Yadav Y, Singh A, Association of SARS-CoV-2 with renal complications: A review. (Submitted in BMC nephorology).
  41. Choudekar A, Singh A. SARS-CoV2 Vaccines: A Legacy of efficacies. (Submitted in Heliyon).
  42. Kumar Y, Singh A. Proteomics research in Juvenile Idiopathic Arthritis (JIA): Towards proteome guided clinical diagnosis and treatment management. (Submitted in Expert reviews of proteomics).
  43. Singh S, Gopinath K, Singh A, Sood R, Rattan A. Mycobacterium tuberculosis.in book Essential of tuberculosis in children 5th Edition (2022), Jaypee Medical Publisher (P) LTD  edited by Dr. S.K. Kabra.
  44.  Singh S, Gopinath K, Singh A, Rattan A. Nontuberculous mycobacterium. in book Essential of tuberculosis in children 5th Edition (2022), Jaypee Medical Publisher (P) LTD  edited by Dr. S.K. Kabra.
  45. Sriastava S, Singh A, Sharma P. (2022). Point of care (POC) detection technique for mycobacterium. In book entitled “Diagnosis of Mycobacterium” (Submitted Springer Nature).
  46. Singh A, Sehgal T, Datta S. (2022). Assessment of Hematological and Biochemical Parameters in the Diagnosis and Prognosis of Tuberculosis in book entitled “Diagnosis of Mycobacterium” (Submitted Springer Nature).
  47. Gupta AK, Singh A. (2022). Diagnosis of Zoonotic tuberculosis in book entitled “Diagnosis of Mycobacterium” (Submitted Springer Nature).
  48. Kumar B, Gupta AK, Singh A. (2022). Hepatitis-B Immunopathogenesis: Role of Cytokines in Disease Progression. In book entitled “Cytokine Response Against Major Human Viral Infections” published by Elsevier.

BOOKS

49. A book entitled “Diagnosis of Mycobacterium” as Editor in Nature Springer. (communicated).

50. A book entitled “Therapeutics of Mycobacterium” as Editor in Nature Springer. (communicated).

51. A book entitled “Vaccines for Mycobacterium” as Editor in Nature Springer. (communicated).

WHOLE GENOME SUMBISSION

Leishmania sp. AIIMS/LM/SS/PKDL/LD-974 Genome sequencing, GeneBank Accession No. LBGS00000000.

Research Guidance

Ph.D. positions are available for students with their own JRF fellowships in the department. 

Conference/ Workshops/Seminars Attended

PAPER PRESENTATION 

  1. Singh A, Gupta AK, Singh S. “Evaluation of point-of-care test for detection of pulmonary and extra-pulmonary tuberculosis cases directly from patient blood samples” ASM Microbe 2020, Chicago, USA. June 18-22, 2020.
  2. Singh A, Gupta AK, Singh S. “Development of point-of-care detection method for pulmonary and extra pulmonary tuberculosis” International Meeting on Emerging Disease and Surveillance (IMED-2018), Vienna, Austria, November 9-12, 2018.
  3. Gupta AK, Singh A, Sharma S, Singh S. “Immuno-diagnostic test for active tuberculosis”.  International Meeting on Emerging Disease and Surveillance (IMED-2018), Vienna, Austria, November 9-12, 2018.
  4. Singh A, Gupta AK, Singh S. Development of point-of-care diagnostic test for detection of Mycobacterium tuberculosis directly from patient blood samples” Laboratory Medicine Congress and Exhibition-      2018 (LMCE-2018), Seoul, South Korea, November 1-3, 2018.
  5. Gupta AK, Singh A, Sharma S, Singh S. “A rapid immuno-diagnostic test for TB”. Laboratory Medicine Congress and Exhibition- 2018 (LMCE-2018), Seoul, South Korea, November 1-3, 2018.
  6. Gupta AK, Srivastava S, Singh A, Singh S. Post kala-azar dermal leishmaniasis: is it a result of endosymbiotic infection of leishmania with Parvibaculum lavamentivorans 6th World Leish, Toledo, Spain, May 16th - 20th 2017.
  7. Srivastava S, Mishra J, Gupta AK, Singh A, Mishra PS, Singh S. “Emergence of miltefosine resistant cases of visceral leishmaniasis in India” 6th World Leish, Toledo, Spain, May 16th - 20th 2017.
  8. Singh S, Gupta AK, Srivastava S, Singh A, Shankar P. “Superinfection with an endosymbiotic bacterium Parvibaculum lavamentivorans leads to manifest Leishmania donovani as host kala azar dermal leishmaniasis.” 26th ECCMID, at Amsterdam, Netherland, April 9th - 12th 2016.
  9. Gupta AK, Singh A, Sharma P, Singh S. Discovering Novel Antigens for the Rapid Diagnosis and Prognosis of Tuberculosis. ASM Conference on Innovative Microbial Ecology for Mitigation of Antibiotic Resistance and Bacterial Diseases, Arlington,USA, March 22-25, 2017.
  10. Singh A, Gupta AK, Gopinath K et al., “Comparative proteomic analysis of sequential isolates of Mycobacterium tuberculosis sensitive and resistant Beijing type from a patient with pulmonary tuberculosis”. Presented at 2nd Asian African Congress of Mycobacteriology (AASM 2017), from 25-28 February 2017 in Isfahan, Iran.
  11. Gupta AK, Saumya S, Singh A, Singh S. “Development of rapid immuno-diagnostic test for the early detection of tuberculosis”. Presented at 2nd Asian African Congress of Mycobacteriology (AASM 2017), from 25-28 February 2017 in Isfahan, Iran.
  12. Singh A, Gupta AK, Gopinath K, Singh S. “Identification of novel point-of-care biomarkers: validation and evaluation for the rapid detection of TB and MDR-TB” presented at Microbe 2016 at Boston, USA on 16-21 June, 2016.
  13. Singh A, Gupta AK, Singh S, “Identification of novel biomarkers for rapid diagnosis of multidrug resistant tuberculosis”. Poster presented at Global Healthcare Summit 2016 (GHS 2016) research paper competition by AIIMS-AAPI. December 29, 2015 at AIIMS, New Delhi, India.
  14. Singh A, Gupta AK, Gopinath K, Sharma P, Singh S “Preparation of novel recombinant antigens for the rapid diagnosis of drug resistant tuberculosis” accepted in ICAAC/ICC 2015 (ASM). September 17-21, 2015, San Diego, California, USA.
  15. Singh A, Gopinath K, Sharma P, Sharma P, Bisht D, Singh S “Comparative proteomic analysis of sequential isolates of Mycobacterium tuberculosis Beijing genotypes from a patient with pulmonary tuberculosis turning from drug sensitive to multidrug resistant” accepted in World Congress on Infectious Disease 2015 International conference (Omics Group). August 10-12, 2015, London, UK.
  16. Rufai SB, Singh A, Kumar P, Singh S, Singh S. Performance of Xpert MTB/RIF assay for detection of Mycobacterium tuberculosis from pleural fluid samples” at IAMM Delhi Chapter conference at Vardhman Mahavir Medical College & Safdarjung Hospital, New Delhi on 25th April, 2015
  17. Singh A, Gopinath K, Sharma P, Bisht D, Sharma P, Singh S “Proteomic analysis of sequential isolates of Mycobacterium tuberculosis from a patient of drug resistant pulmonary tuberculosis” In FEMS 2013 5th congress of European Microbiologist (FEMS-2013), 21 to 25th July, 2013 (FEMS Young scientist award).
  18. Singh J, Kumar S, Sankar MM, Singh A, Kumar H, Singh N, Kalaiwani M, Singh S “Incidence and prevalence of tuberculosis among household contacts of pulmonary tuberculosis patients in a Peri-urban population of South Delhi, India.” In zoonotic mycobacterial infections and their impact on public health, 25th to 27th February, 2013, AIIMS, New Delhi.
  19. Singh A, Gupta AK, Sharma P, Singh S. “Development of strip-based assay for the diagnosis of tuberculosis directly from patient serum” "Clinical microbiology: current challenges & future directions". In XXXVI National conference of Indian Association of Medical Microbiologist (MICROCON 2012), 22nd to 25th November, 2012, New Delhi
  20. Singh A, Gupta AK, Singh J, Sharma P, Singh S. “Cloning, expression, purification & evaluation of a novel recombinant antigen for rapid serodiagnosis of tuberculosis” In 5th congress of Federation of Immunological Societies of Asia-Oceania (FIMSA 2012), 14 to 17th March 2012, New Delhi, India.
  21. Gopinath K, Singh A, Singh S. “Comparative genomic analysis of ATP Binding cassette (ABC) transporter genes of M. tuberculosis and M. avium subp. paratuberculosis” in FEMS 2009 3rd congress of European microbiologist ‘microbes and man- independent and future challenges’ at Gothenburg, Sweden on 28th June to 2nd July 2009.
  22. Singh A, Gopinath K, Sharma P, Katoch VM, Singh S. “Proteomic analysis of M. tuberculosis during the stage of resistance to isoniazid and rifampicin” in IX Sir Dorabji Tata Symposium at National Institute of Advanced Studies, Indian Institute of Science campus, Bangalore, India on 10-11th March, 2008. (Best Poster award)
  23. Gopinath K*, Singh A*, Singh S “ATP binding cassettes transporters of M. tuberculosis and M. avium paratuberculosis phylogeny to regulatory functions” in International Conference on Opportunistic Pathogens in AIDS, January 27th-29th 2008 at All India Institute of Medical Sciences, New Delhi, India (Best Poster Award) *authors contributed equally.
  24. Gopinath K*, Singh A*, Singh S “ATP binding cassettes transporters of M. tuberculosis and M. avium paratuberculosis” in XXXI National Congress on Indian Association of Medical Microbiologists from 16-18th November, 2007 at Mangalore, India. *authors contributed equally.

CERTIFICATION/TRANING/ COURSES

  1. Certificate course on “Monkeypox: Epidemiology, preparedness and response for African outbreak contexts” by World Health Organization (WHO) on 10th August, 2022.
  2. Certification course on “Fundamentals of working safely in a biological safety cabinet” by US Division of laboratory systems, CDC, Atlanta on 5th May, 2022.
  3. Training program on “Good Clinical Practice (GLP)” organized by the Clinical Development Services Agency (CDSA), a center of Translational Health Science & Technology Institute (THSTI), an autonomous institute under DBT in collaboration with the All India Institute of Medical Science (AIIMS), New Delhi.CRU-AIIMS & CDSA, THSTI on dated from 9th Feb, 2022 to 2nd March, 2022.
  4. Certification course on “Biosecurity for clinical laboratories” by Institute of Public Health Practice University of Iowa on Nov 18,2021.
  5. Certification on ‘Basic Course in Biomedical Research” as mandated by the National Medical Commission (NMC) organized by Indian Council of Medical Research (ICMR) and National Institute of Epidemiology (NIE), March-June 2021.
  6. Training on “systematic literature review and meta-analysis” and obtained A+ grade on one-week faculty development programme organized by Teaching Learning Centre, Ramanujan College, University of Delhi, Under Ministry of Education. From 10-16th August, 2021.
  7. Training Program on Introductory Course on Research Methodology & Biostatistics under Dr. Padam Singh Research & Development Scheme organized by ICMR-National Institute of Medical Statistics (NIMS) from 24.08.2020 to 25.09.2020. Ref. No. Ref: DCS/IAS/R&D/2020/348.
  8. Certificate course on“covid-19: operational planning guidelines and covid-19 partners platform to support country preparedness and response” by World Health Organization (WHO) on 4th April, 2020.
  9. Certificate course on “Severe Acute Respiratory Infection (SARI) Treatment Facility Design” by Completed World Health Organization (WHO) on 4th April, 2020.
  10. Course on the topic “Coronavirus Disease 2019” by AACC Learning Lab for Laboratory Medicine on NEJM Knowledge+” on 18th March, 2020.
  11. Research training on intracellular pathogens workshop jointly conducted by ICGEB & NIH, at ICGEB. New Delhi on 28th February-1st March, 2012.

SYMPOSIUM/WORKSHOP/WEBINAR

  1. Attended 4th edition of India Droplet Digital PCR Symposium 2021 organized by Bio-Rad, India on dated 23rd Nov to 25th November, 2021.
  2. Attended 10th Annual CME of clinical biochemistry-2021 “Therapeutic drug monitoring-the right test at the right time” organized by Department of Biochemistry, Sir Ganga Ram Hospital, New Delhi and ACBI-Delhi Chapter on 16t November, 2021.
  3. Attended two-day workshop on ‘Research Methodology And Publication Ethics’ organized by B.R. Ambedkar Central Library, Jawahar Lal Nehru University, New Delhi on Oct 22-23, 2020
  4. Organized and chaired session on ‘The 2nd HeriCure-Ukzn: A hybrid International conference and webinar on “Recent progress in bioactive compounds & molecules against respiratory disease and comorbidities: theranostics and future challenges” 15-16 May, 2021.
  5. Participated in “SPARC-INDO-US Immunology Workshop” Jointly Organized by Indian Institute of (IIT) Ropar, India & The George Washington University, USA on June 12th-13th, 2020.
  6. Attended the webinar hands-on tutorial: workflow advances for whole cell correlative cryo-microscopy on 2nd July, 2020.
  7. Attended the webinar mass spectrometry past and present - emerging technologies and strategies for quality management in Today's Clinical Laboratory on June 26, 2020.
  8. Attended 5th Bio-Rad ddPCR asia pacific virtual symposium (Week 2: cell & gene therapy session 1) organized by Bio-Rad on 13th May, 2020.
  9. Participated in World Health Organization course “Infection Prevention and control (IPC) for Novel Coronavirus (Covid-10)” on 4th April, 2020.
  10. Organized CCRF hands-on-workshop Series I “Polymerase Chain Reaction (PCR), RT-PCR and Real-Time PCR technique: Application in diagnostics and research at CCRF, AIIMS, New Delhi on 27-28 February, 2020.
  11. Organized symposium on challenges in diagnostics and treatment of tuberculosis “Its time…to get ready for world TB day 2019, at Department of Microbiology, AIIMS Bhopal. 24th March, 2019.
  12. Attended Pre-conference workshop “Recent advances in molecular biology and imaging technique” on 10th October, 2019 at AIIMS, Bhopal.
  13. Organized “Hands on workshop on antimicrobial susceptibility testing by broth micro dilution and polymyxin NP test with WHONET for antibiogram” on 25th-30th April at AIIMS, Bhopal.
  14. Attended World Immunology Day Symposium on “Gazing in to the horizon if immune system” on 1st May, 2018 at AIIMS, New Delhi.
  15. Participated in the “1st National workshop on protein purification and characterization” held at University of Delhi South Campus organized by Centre for Innovation in Infectious Disease Research, Education and Training (CIIDRET) on 6-7 February, 2018.
  16. Participated in workshop on Access and Availability of Medical Literature in Electronic Environment (AMLEE-2018) organized by Dr. B.B. Dikshit Library, AIIMS, New Delhi on 16th April. 2018 at AIIMS, New Delhi.
  17. Participated as Organizing Committee of SYSCON 2017-2018 ‘Bridging the gap basic and clinical research from bench to bedside’ organized by society of young scientist, AIIMS, New Delhi from 11th-12th January, 2018.
  18. Participated in the ‘Wanted Leaders for a TB-free world held on 21-22 March, 2018 at AIIMS, New Delhi, India.  
  19. Participated in 1st Healthy food symposium organized by CSI, Delhi Branch under the Aegis of AIIMS, New Delhi on 29th September, 2017 at AIIMS, New Delhi.
  20. Attended workshop “Molecular detection of respiratory viruses” at National Institute of Virology, Pune, India, 22nd September to 26th September 2016.
  21. Participated in the ‘World Immunology Day’ symposium on Immuno-prophylaxis and immunotherapy held on 30th April, 2016 at AIIMS, New Delhi, India.
  22. Participated in ASM CME cum workshop on emerging infections in India organized by The American Society for Microbiology (ASM) and All India Institute of Medical Sciences at AIIMS, new Delhi on 21st April, 2016.
  23. Participated in National symposium on ‘Challenges in tuberculosis diagnosis and treatment’ organized by AIIMS, New Delhi on 30th March 2016.
  24. Participated as speaker and resource person in symposium “AIIMS-DBT brain storming symposium on challenges in tuberculosis diagnosis and management” on World TB day, 24th March, 2015.
  25. Organized TB awareness programme at AIIMS, New Delhi on 24th March, 2014 in collaboration with BD Diagnostic India.
  26. Participated in the symposium ‘Find Treat Cure TB’ held at AIIMS, New Delhi on 24th March, 2014.
  27. Participated in the computer skill development workshop series organized by K.L. wig Centre for Medical Education and Technology (CMET), AIIMS, New Delhi on January-March, 2014.
  28. Participated in symposium on ‘Stop tuberculosis in my lifetime’ organized by AIIMS, New Delhi on 26th March, 2013.
  29. Attended Workshop “XXXVI National Conference of IAMM” as resource person, 22nd to 25th November, 2012 at AIIMS, New Delhi, India.
  30. Workshop on “Improving tuberculosis care and control” 4th November, 2011 at AIIMS, New Delhi, India.
  31. Symposium on “World TB Day symposium”, 29th March, 2011 at Department of Microbiology, AIIMS, New Delhi, India.
  32. Attended Seminar on “frontiers in structural bioinformatics”, 23rd June, 2011 at AIIMS, New Delhi, India.
  33. Workshop “The innovative technique; Real-Time PCR” 2nd May, 2008 at Vardhman Mahaveer Medical College & Safdarjung Hospital, New Delhi, India.
  34. Organize in “International Conference on Opportunistic Pathogens” (ICOPA-INDIA, 2010), September 28-30, 2010 at AIIMS, New Delhi, India.
  35. Participated in “SYS Lecture Series 2010” 28th June, 2010, organized by Society of Young Scientist, AIIMS, New Delhi, India 
  36. Attended “Current trends in structural biology’ 2010” 30th March, 2011 at Department of Biophysics, AIIMS, New Delhi, India.
  37. Attended Second National Conference of “Indian Academy of Tropical Parasitology (TROPACON-2) with pre-conference CME on opportunistic human parasites in AIDS & post conference workshop on laboratory diagnosis of opportunistic intestinal parasites”, 30th October to 2nd November, 2008 at AIIMS, New Delhi, India.
  38. Organized ‘International Conference on Opportunistic Pathogens in AIDS (ICOPA-INDIA-2008)’, January 27-29, 2008 at AIIMS, New Delhi, India.
  39. Attended “Seminar-cum-workshop on right to information” 27th, May 2007 at AIIMS, New Delhi, India.
  40. Attended “International Conference on Opportunistic Pathogens in AIDS (ICOPA-INDIA, 2006 & LABCON-3), March 27th -29th, 2006 at AIIMS, New Delhi, India.

PARTICIPATION IN OTHER ACTIVITY

  1. Participated in National conclave on healthcare at constitution club of India organized by Samruddha Bharat on 29th July, 2018.
  2. Participated in 5km Run-2018, run for cleanliness, run for road safety organized by RDA, AIIMS, New Delhi on 14th April, 2018 at AIIMS, New Delhi
  3. Participated in 4km World TB Day Marathon on 24th March, 2015 at AIIMS, New Delhi.
  4. Participated in the RDA AIIMS 5K run organized by RDA AIIMS and Gymkhana in association with Cankids and core diagnostics at AIIMS, New Delhi on 15th February, 2015.
  5. Completed flying at 160kmph form one kilometer at Jumping Height, Rishikesh, India on 11th May, 2014.
  6. Completed bungy jumping from a height of 83 meters with only a rubber cord at jumping height, Rishikesh, India on 11th May, 2014.
  7. Participated as captain of Kho-Kho team at university level from Govt. New Science college in Year 2002-2003.
  8. Participated in chess competition at Government of Madhya Pradesh, higher education department at district level in 2002-2003
  9. Participated in quiz competition on nature and environment and secure second rank at Govt. New Science College, APS, University, Rewa in Year 2001-2002.
  10. Works as President of cultural and literacy student union society at Govt. New Science College, APS, University, MP in Year 2001-2002.
  11.  Participated in chess competition at Government of Madhya Pradesh, higher education department at district level in Year 2001-2002.
  12. Participation in youth festival competition and secured first prize organized by Govt. New Science College, APS University, Rewa MP.
  13. Participated in 5th M.P. state invitation Tae-Kwon-Do organized by Jabalpur Tae-Kown-Do Association and Jabalpur Tae-Kwon-Do club from 29th-31st October, 1998.

GENEBANK SUBMISSION (n=26)

  1. Mycobacterium tuberculosis isolate TB-2016-I-SP eis gene, promoter region. KF279682.
  2. Mycobacterium tuberculosis isolate TB-2016-II-SP eis gene, promoter region. KF279683.
  3. Mycobacterium tuberculosis isolate TB-2016-III-SP eis gene, promoter region. KF279684.
  4. Mycobacterium tuberculosis isolate TB-2016-IV-SP eis gene, promoter region. KF279685.
  5. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-1920/06/SP cfp17 gene, partial cds. KC147003 (Rv1827).
  6. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-1920/06/SP DIV IV family protein (wag31) gene, complete cds. KC147004 (Rv2145c).
  7. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-1920/06/SP Rv0164-like gene, complete cds. KC147005 (Rv0164).
  8. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-1920/06/SP phosphoglycerate kinase (pgk) gene, complete cds. KC147006 (Rv1437).
  9. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-1920/06/SP Rv2714-like protein gene, complete cds. KC147007 (Rv2714).
  10. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-1920/06/SP lipase/esterase (lipN) gene, complete cds. KC147008 (Rv2970c).
  11. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-2016-I/05-SP hypothetical protein gene, complete cds. JX987082 (Rv2714).
  12. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-2016-I/05-SP putative phosphoglycerate kinase (pgk) gene, complete cds. JX987083 (Rv1437).
  13. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-2016-I/05-SP putative lipase/esterase (lipN) gene, complete cds. JX987084 (Rv2970c).
  14. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-2016 I/05 SP hypothetical protein (TB18.1) gene, complete cds. JQ885575 (Rv0164).
  15. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-2016 I/05 SP GarA (garA) gene, complete cds. JQ885576 (Rv1827).
  16. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-2016 I/05 SP DIVIVA family protein Wag31 (wag31) gene, complete cds. JQ929302 (Rv2145c).
  17. Mycobacterium intracellulare strain AIIMS/LM/SS/TB-4582/09/PF 16S ribosomal RNA (rrs) gene, partial sequence. JQ037845 (16s RNA).
  18. Mycobacterium lentiflavum strain AIIMS/LM/SS/TB-5734/10/AF 16S ribosomal RNA (rrs) gene, partial sequence. JQ037846 (16s RNA)
  19. Mycobacterium simiae strain AIIMS/LM/SS/TB-5787/10/EA 16S ribosomal RNA (rrs) gene, partial sequence. JQ037847 (16s RNA).
  20. Mycobacterium simiae strain AIIMS/LM/SS/TB-6467/10/CU 16S ribosomal RNA (rrs) gene, partial sequence. JQ037848 (16s RNA).
  21. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-5518/10/AF 16S ribosomal RNA (rrs) gene, partial sequence. JQ037849 (16s RNA).
  22. Mycobacterium arosiense strain AIIMS/LM/SS/M-804/10/SP 16S ribosomal RNA (rrs) gene, partial sequence. JQ037850 (16s RNA).
  23. Mycobacterium tuberculosis strain AIIMS/LM/SS/TB-5518/10/AF 16S ribosomal RNA (rrs) gene, partial sequence. JQ037849 (16s RNA).
  24. Mycobacterium arosiense strain AIIMS/LM/SS/M-804/10/SP 16S ribosomal RNA (rrs) gene, partial sequence. JQ037850 (16s RNA).
  25. Leishmania donovani miltefosine transporter gene, partial cds. KX827627.
  26. Leishmania donovani strain DD8 kinesin-like protein (kin) gene, partial cds. KM265138.
Conference/ Workshops/Seminars Organised
Organized and chaired various National and International conference, workshop and symposium
  1. Organized and chaired session on ‘The 2nd HeriCure-Ukzn: A hybrid International conference and webinar on “Recent progress in bioactive compounds & molecules against respiratory disease and comorbidities: theranostics and future challenges” 15-16 May, 2021.
  2. Organized CCRF hands-on-workshop Series I “Polymerase Chain Reaction (PCR), RT-PCR and Real-Time PCR technique: Application in diagnostics and research at CCRF, AIIMS, New Delhi on 27-28 February, 2020.

  3. Organized symposium on challenges in diagnostics and treatment of tuberculosis “Its time…to get ready for world TB day 2019, at Department of Microbiology, AIIMS Bhopal. 24th March, 2019

  4. Organized “Hands on workshop on antimicrobial susceptibility testing by broth micro dilution and polymyxin NP test with WHONET for antibiogram” on 25th-30th April at AIIMS, Bhopal.

  5. Participated as Organizing Committee of SYSCON 2017-2018 ‘Bridging the gap basic and clinical research from bench to bedside’ organized by society of young scientist, AIIMS, New Delhi from 11th-12th January, 2018

  6. Organized TB awareness programme at AIIMS, New Delhi on 24th March, 2014 in collaboration with BD Diagnostic India.
Peer Recognition

EDITOR

  1. PNAS, USA (Guest Review Editor)
  2. Frontiers in Clinical Microbiology (Guest Associate Editor)
  3. Canadian Journal of Infectious Disease and Medical Microbiology (Academic Editor)
  4. Current Indian Science 

 

EDITORIAL BOARD MEMBER

  1. Journal of Health and Medical Research
  2. Journal of Proteomics, Bioinformatics, and Genomics
  3. Tuberculosis: Research and Treatment
  4. Advanced Techniques in Clinical Microbiology
  5. American Journal of Pulmonary and Respiratory Medicine

REVIEWER

  1. PLoS One
  2. Frontiers Medicine
  3. Frontiers Microbiology
  4. Frontiers in Public Health
  5. Frontiers in Cellular and Infection Microbiology
  6. Frontiers in Immunology
  7. Frontiers Clinical Microbiology
  8. BMC Microbiology
  9. Cureus
  10. BMC Infectious Disease
  11. PLOS Global Public Health
  12. Tuberculosis & Respiratory Disease (TRD)
  13. International Journal of Molecular Sciences (MDPI)
  14. Tropical Medicine and Infectious Disease (MDPI)
  15. Metabolites (MDPI)
  16. Molecules (MDPI)
  17. Biomedical and Biotechnology Research Journal (BBRJ)
  18. International Journal of Mycobacteriology
  19. Open Journal of Clinical and Medical Case Reports
  20. Journal of Laboratory Physician
  21. Tuberculosis Research & Treatment
  22. Global Journal of Infectious Diseases and Clinical Research
  23. Journal of Pediatric Intensive Care
  24. Journal of Drug Research in Ayurvedic Sciences
  25. Open Biology, Royal Society of Chemistry
Invited Talks/ Resource Person
1. Invited talk and resource person in the symposium “AIIMS-DBT brain storming symposium on challenges in tuberculosis diagnosis and management” on World TB day, 24th March, 2015.
Patent Filed/ Published
PATENT FILED

Singh S, Amit Singh, Gopinath K. Novel Protein Markers of Drug Resistance in Mycobacterium tuberculosis.

Contribution Towards Institutional Building
Helps in writing proposal for creation of Translational Medicine Centre at AIIMS, Bhopal, Madhya Pradesh, India
Citations

Total Citation 1000 (2022)

Innovations
Development of immunoassay for detection of Mycobacterium tuberculosis directly from patients blood samples
Community Participation
Life member of Being social foundation and ISET foundation where we routinely organized awareness programme and help camps for needy.
Advisory Member and Life member of SETF
Executive Committee Member of APMS
Other Significant Achievements

CERTIFICATION/TRANING/ COURSES (n=11); WGS (n=1); Genebank submission (n=26)